Biomolecular Recognition

We’re interested in the structural determinants of molecular recognition in complexes of proteins with recognition agents such as monoclonal antibodies and aptamers. Our primary techniques are expression, mutagenesis, fluorescence anisotropy (kinetics) and titration calorimetry. Topics of current interest include the recognition of hen egg lysozyme by a “homologous series” of antibodies differing in combining site rigidity and cross-reactivity (with S. Smith-Gill of NIH), and the biophysical chemistry of aptamer/protein recognition (with A. Ellington of UT-Austin).